Wound healing process involves a range of intracellular and extracellular reactions. One way to enhance and optimise this process in both hard and soft tissues is by way of platelet concentrates called Concentrated Growth Factors, CGF.
Platelets contain high quantities of growth factors such as platelet derived growth factor, (PDGF), transforming growth factor-B1 (TGF-b1) and (TGF-b2), fibroblast growth factor (FGF), vascular epithelial growth factor (VEGF), and insulin like growth factor (IGF). All these stimulate cell proliferation, matrix remodelling and angiogenesis which are vital to the healing process.
Our aim is to accelerate this process by applying techniques using platelet concentrates to obtain different ratios of platelets, growth factors, leukocytes and fibrin matrix. The first generation of platelet concentrate rich plasma (PRP) was used to accelerate healing but with limitations. Research later indicated its poor ability to induce cell proliferation and healing.
Platelet Rich Fibrin (PRF), a second generation platelet concentrate developed by Choukroun, allowed the formation of a solid fibrin consistency that consisted of a significantly higher concentration of platelets, leukocytes and growth factors that greatly aided the healing process.
Concentrated Growth Factors (CGF) was developed by Sauo 2006 and was the third generation platelet concentrate. This involved centrifuging venous bloods with the use of a special device (Medifuge) without the use of additives in the blood collection tubes, then spinning the blood at variable speeds, (2400-2700 rmps). This isolates a production of a much larger and denser fibrin matrix that is richer and superior in growth factors. This in turn allows for a superior regenerative capacity and has a larger versatility in application of wound healing.
The growth factors in CGF are many as described above PDGF, EGF, TGF-b1, TGF-b2, TGFa, VEGF, FGF, Fibrinogen and Stem Cells. Our body contains undifferentiated, primitive, precursor cells including those that regenerate/repair both hard and soft tissues. These cells are designated as CD34T cells. These play a vital and important role in our field as they do not only aid in healing but applied in different locations they can stimulate bone formation (bone grafting with AFG sticky bone, sinus lifts) and soft tissue healing.
We apply both CGF/AFG and similar products in preference to allografts (grafts from cadavers) or xenografts (grafts from other animals, such as cows or pigs). This allows the patient to have both natural and autogenous products in both soft and hard tissue grafting procedures. This results in a more rapid and non-inflammatory reaction by the hosts tissues.
My Tooth Bone
My Tooth Bone is the latest technology in dental bone grafting. An extracted tooth from the patient can be used and noble bone graft material after and appropriate preparation and decalcification process in either a block or powder form. This preparation of a fresh autogenous demineralised tooth is a great alternative option rather than the use of an autogenous donor site or alogenous graft materials.
This involves the use of a patient’s own tooth to be used as a source of bone graft with the use of the Vacua – Sonic System. Similar to bone a tooth consists of organic matter of Type 1 collagen and minerals of hydroxyl Apatite (HA). The main difference is that Type 1 collagen of teeth is denser. A substance called DPP (Dentine Phosphophoryn) which is contained in the Type 1 collagen helps bone generation by increasing the activity of BMP and DMP-1 in the calcification of tissues.
Unlike bones, the HA of teeth is highly crystalline which does not promote osteoconduction. This requires the processing performed with the Vacua Sonic system. The Vacua Sonic rapidly demineralises the tooth HA into a low crystalline HA and effective proteins that are found in dentine.
This promotes attachment of oesteoblasts to the graft material and a process of remineralisation. The Vacua Sonic process is rapid and renders an autogenous bone graft that is 100% sterile.
An extracted tooth from the patient has many advantages:
- No risk of cross contamination from allograft material.
- Faster bone regeneration.
- Similar to biochemistry to human bone.
- Chairside preparation at time of extraction.
- Patients can extract and store their whole tooth for up to 2 months, or they can process their teeth in power or block form and store them up to 6 months and still have the osseoinduction and osseoconduction quantities still present.
Contact us to find out more about your options when it comes to using Stem Cells for Dental Implants
Contact us on (03) 9887 0888 or book an appointment online to find out more about your options using stem cell technology.